Figure 3.

Electron-microscopic localization of KCC2 protein in the rat SN. A, B, Electron microscopy revealed that TH-immunopositive dendrites (DAB labeled) do not contain KCC2 protein (gold particles). Immunoreactivity for TH was found in dopaminergic dendrites, whereas KCC2 was localized in the dendritic membrane of nondopaminergic neurons. KCC2 immunolabeling (arrowheads) was often observed in the vicinity of symmetric synapses (arrows) at the postsynaptic site. Scale bars, 500 nm. b, Bouton; d, dendrite.