Figure 6.

Confocal microscopic images of double-immunostained sections show that TH-immunoreactive dopaminergic neurons express ClC-2 in the SNc. A, Immunofluorescence staining against TH visualizes the dopaminergic neurons in the SNc (green). Both perikarya and dendrites display TH immunoreactivity. B, In the same section, ClC-2-immunopositive cells were identified on the basis of the presence of a red fluorescent signal. Similarly, immunolabeling was observed selectively in perikarya and proximal dendrites of dopaminergic neurons. Scale bars, 25 μm.