Figure 2.

Ca²⁺ dependency of the Tat-induced release of [³H]ACh from human (A) and rat (B) cortical synaptosomes. Synaptosomes were staffed with BAPTA or heparin during cortical tissue homogenization, whereas antagonists or Cd²⁺ ions were added during superfusion, 8 min before Tat. At the concentrations used, these drugs did not modify, on their own, the [³H]ACh release (data not shown). Results are expressed as the percentage of increase over basal release; data are means ± SEM of 3-12 experiments run in triplicate. ^*p < 0.05 versus respective control; ^**p < 0.01 versus respective control.