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. 2003 Jul 30;23(17):6914–6927. doi: 10.1523/JNEUROSCI.23-17-06914.2003

Figure 8.

Figure 8.

Treatment of neurons with antibody 22C11 causes apoptosis and DNA synthesis and expression of C57 or C31 causes neuronal apoptosis and DNA synthesis. A, Sustained treatment of neurons with 1 μl/ml of 22C11, a ligand mimetic for APP, causes neuronal apoptosis. Expression of either myc-PAK3-K297R or peptide myc-PAK3 (101-126) significantly abrogates 22C11-mediated neuronal apoptosis. Significant differences were confirmed by ANOVA (F(6,62) = 28.0; p < 0.0001). Post hoc t tests done using the Bonferroni multiple comparisons test reveal significant differences between the following groups: 22C11 or 22C11+lac versus 22C11+mycPAK3K297R, 22C11+mycPAK3 (101-126), 22C11 versus lac (no 22C11), mock (no 22C11) or 9E10 (p < 0.001 for all comparisons). B, Sustained treatment of cortical neurons with 22C11 leads to a significant increase in the number of neurons showing DNA synthesis. Significant differences were confirmed by ANOVA (F(5,53) = 95.4; p < 0.0001). Post hoc t tests done using the Bonferroni multiple comparisons test reveal significant differences between the following groups: 22C11 (1 μl) or 22C11 (2 μl) versus 9E10 (1 μl), 9E10 (2 μl), lac, or mock (p < 0.001 for all comparisons), and 22C11 (1 μl) versus 22C11 (2 μl) (p < 0.01). All other relevant comparisons were NS. C, Infection of neurons with HSV vectors expressing myc-tagged C57 or C31 causes an increase in the number of apoptotic nuclei relative to infection of neurons with control HSV-Lac. Coexpression of dominant-negative PAK3 or addition of PTX causes a significant reduction in the number of apoptotic nuclei in cultures expressing myc-C57 or myc-C31. Significant differences were confirmed by ANOVA (F(8,81) = 34.95; p < 0.0001). Post hoc t tests done using the Bonferroni multiple comparisons test reveal significant differences between the following groups: mycC57 versus mycC57+mycPAK3K297R, mycC57+PTX or lac, and mycC31 versus mycC31+mycPAK3K297R, mycC31+PTX or lac (p < 0.001 for all comparisons). Other relevant comparisons were NS. D, Expression of myc-tagged C57 or C31 leads to a significant increase in the number of neurons showing DNA synthesis. Significant differences were confirmed by ANOVA (F(3,36) = 12.98; p < 0.0001). Post hoc t tests done using the Bonferroni multiple comparisons test demonstrated a significant increase in the number of BrdU-positive nuclei in cultures infected with HSV-myc-C57 or HSV-myc-C31 versus lac or mock (p < 0.001). Other relevant comparisons were NS.