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. 2003 Aug 20;23(20):7592–7601. doi: 10.1523/JNEUROSCI.23-20-07592.2003

Figure 7.

Figure 7.

The LI and the entire set II are required for forming the internal clusters. a, Schematic representation of GFP-tagged ABP fragments. GFP-tagged ABP fragments, GFP-LI-SII and its deletion mutants, GFP-LIΔ80-SII, GFP-LIΔ100-SII, GFP-SII, and GFP-LI-PDZ4,5, were expressed in hippocampal neurons as indicated. The presence of the internal clusters is indicated by a plus (+), whereas the absence of the cluster is labeled as minus (-). b, ABP fragments were expressed in hippocampal neurons as indicated. After 24 hr of transfection, GFP-tagged protein was visualized by fluorescence with confocal microscopy. GFP-LI-SII and GFP-LIΔ80-SII formed internal clusters (A, B), whereas GFP-SII, GFP-LIΔ100-SII, and GFP-LI-PDZ4,5 were diffusely distributed in cell body, dendrites, and spines (C-E). Scale bar, 20 μm. c, Sequence comparison of the distal 40 amino acids of linker I in ABP and GRIP. Linker I of ABP has 120 amino acids, whereas that of GRIP has 135 amino acids. The aa 80-100 region of linker I of ABP is required for membrane association of the ABP membrane binding domain and contains a subregion rich in basic residues (underlined).