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Method of sensory deprivation of the tail. A, Sensory deprivation of the tail was achieved by attaching a tube to a plastic ring glued to the ventral side of the proximal tail. The tube was either empty or filled with salve. B, A modified tube with two compartments separated by a membrane was used to study possible systemic effects of EMLA. Note that the plastic ring was glued more proximal on the tail than in A. The proximal compartment was filled with EMLA.
