Figure 3.

Blocking effect of intra-DRN microinjection of metergoline (MET) on sleep deprivation-induced 5-HT release in the SCN. Consistent with our previous published results, sleep deprivation caused ∼150% increase in SCN 5-HT release. This was completely blocked by the metergoline treatment. Inset, Integrated values for 5-HT release as a percentage of pretreatment baseline over the 3 hr sleep deprivation period designated by the horizontal bar. For both graphs, ^ap < 0.05 versus pretreatment baseline levels; ^*p < 0.05 versus vehicle (VEH) controls for a given time point. Data are mean ± SEM (n = 5 per group).