Figure 7.

Reduced levels of synaptic proteins in sciatic nerves of PS1 KI ^M146V mice. Sciatic nerves and spinal cord segments from two WT and two KI animals were dissected and homogenized. Protein (15 mg) was loaded in each lane. Western blots were developed with the indicated antibodies. Note the significant reduction in PS1, APP, and synaptophysin (Syn) in sciatic nerves, but not in the spinal cord, of PS1 KI mice. A reduction in GSK3β-Pser9 and 63–90 immunoreactivity is evident in both sciatic nerve and spinal cord samples from KI mice. No significant changes between PS1 WT and KI samples were detected in the level of total GSK3β, KHC, SNAP25, or tubulin.