Figure 6.

Morphology of microglial cells under LPS, BAPTA, and ionomycin treatment. Microglial cultures were treated for 24 hr with LPS (100 ng/ml), BAPTAAM (50 μm), a combination of BAPTA and LPS, or ionomycin (1 μm). Untreated cultures served as control (CTL). Images demonstrate the appearance of cells in phase-contrast microscopy. During LPS treatment, cells revealed the characteristic gross morphology known for activated cells in vitro, including shape changes, granulation, and membrane ruffling.