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. 2003 Jun 1;23(11):4775–4784. doi: 10.1523/JNEUROSCI.23-11-04775.2003

Figure 5.

Figure 5.

Effect of morphine withdrawal on glutamate uptake in chronic morphine-treated hippocampal neurons and astrocytes. A, B, After morphine withdrawal, the increase in glutamate uptake in cultured neurons was morphine dose (A) and time (B) dependent, whereas no significant change was seen in cultured astrocytes. The glutamate uptake in the morphinetreated group was presented as percentage of that of control group. C, Glutamate uptake in cultured astrocytes could be blocked by PDC but not by DHK treatment and was not significantly altered during morphine withdrawal after 7 d morphine (10 nm) treatment. D, Glutamate uptake in cultured neurons was increased after morphine withdrawal and could be blocked by either PDC or DHK treatment. Glutamate, 10 μm. The control rates in the cultured astrocytes and neurons were 1070 ± 93 and 748 ± 48 pmol · mg -1 · min -1 protein, respectively. The data were presented as mean ± SEM of three independent experiments performed in duplicate. Sal, Saline treatment; Mor, morphine treatment.