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Photobleach dequenching FRET between APP and PS1 demonstrates close proximity between C terminus of APP and loop region of PS1 (A). Cy3-labeled APP (emission, 568 nm) and FITC-labeled PS1 (emission, 488 nm) before and after photobleaching the acceptor (Cy3) in a selected area within the cell. DAPT does not prevent close association of APP with PS1 (B). Scale bar, 10 μm.
