Table 1.
K½ values for cGMP activation
|
|
Phosphorylated |
Dephosphorylated |
||||
|---|---|---|---|---|---|---|
|
|
Control
|
250 nm CaM
|
Control
|
250 nm CaM
|
||
| CNGA1/CNGB1 (n = 6) | 120.6 ± 2.2 μm, nH = 1.9 ± 0.1 | 136.3 ± 3.7 μm, nH = 2.0 ± 0.1 | 59.2 ± 1.0 μm, nH = 1.9 ± 0.1 | 162.2 ± 5.0 μm, nH = 2.0 ± 0.1 | ||
| CNGA1Y498F/CNGB1Y1097F (n = 4) | 75.1 ± 5.3 μm, nH = 2.1 ± 0.1 | 169.0 ± 7.5 μm, nH = 2.1 ± 0.1 | 62.2 ± 1.5 μm, nH = 1.9 ± 0.1 | 155.5 ± 5.9 μm, nH = 2.0 ± 0.1 | ||
| CNGA1/CNGB1Y1097F (n = 13) | 112.2 ± 2.1 μm, nH = 2.1 ± 0.1 | 124.3 ± 5.4 μm, nH = 2.0 ± 0.1 | 56.4 ± 2.6 μm, nH = 1.9 ± 0.2 | 154.3 ± 5.1 μm, nH = 1.9 ± 0.1 | ||
| CNGA1Y498F/CNGB1 (n = 9)
|
132.5 ± 3.4 μm, nH = 1.9 ± 0.1
|
255.3 ± 4.9 μm, nH = 2.0 ± 0.1
|
68.4 ± 3.3 μm, nH = 1.8 ± 0.2
|
169.1 ± 5.8 μm, nH = 1.8 ± 0.2
|
||