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. 2003 Nov 5;23(31):9961–9967. doi: 10.1523/JNEUROSCI.23-31-09961.2003

Figure 1.

Figure 1.

Lmx1b is expressed in serotonergic neurons. Transverse sections through E11.5 pons (A, B), E12.5 caudal pons (C, D), E12.5 medulla (E, F), and E14.5 caudal pons (G-I) are shown. In situ hybridization was performed with the indicated probes (A-F). Lmx1b and Pet-1 are expressed in neurons derived from the ventral midline (A-F, arrows) that correspond to the primordial raphe nuclei. Lmx1b is also expressed weakly in the floor plate (A, arrowhead) and other areas in the medulla (A, star; E, arrowhead). G-H, Coronal sections through the E14.5 pons. Double immunostaining was performed with Lmx1b antibody (G, red) and 5-HT antibody (H, green). I is the superimposed image showing that Lmx1b is expressed in all 5-HT cells (with yellow nuclei) (I, arrow). A small subset of Lmx1b-positive cells is 5-HT negative (I, arrowhead).