Figure 2.
Proinflammatory cytokines affect Aβ secretion. A, Increased Aβ generation in media obtained from N2a cells overexpressing APPsw by proinflammatory cytokines, shown by immunoprecipitation and Western blotting. B, Quantification of total Aβ by immunoprecipitation–Western blotting in five similar experiments as above. C, Analysis of Aβ1–40 and Aβ1–42 secretion in N2a APPsw cells by ELISA (n = 6). Columns represent average ± SEM. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ANOVA, Tukey's post hoc test. c, Control.