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. 2003 Oct 29;23(30):9796–9804. doi: 10.1523/JNEUROSCI.23-30-09796.2003

Figure 8.

Figure 8.

Effect of ibuprofen (IBU) and pioglitazone (PIO) on BACE1 expression, mRNA levels, and activity. A, Increased BACE1 expression in SK-N-SH cells incubated with IFNγ (1 ng/ml) plus TNFα (30ng/ml) is reversed with IBU (10μm) in Western blotting experiments. B, Quantification of BACE1 expression in four to five experiments performed in N2A cells. Cells were incubated with IFNγ (1 ng/ml) plus TNFα (30 ng/ml) with or without IBU (10μm) or PIO(10 μm). C, Modulation of BACE1 steady-state mRNA levels by the same treatments is shown in semiquantitative RT-PCR representation of BACE1 mRNA from SK-N-SH cells. As a negative control, PCR was performed for β-actin with the same samples. D, PPARγ antagonists GW0072X (1 μm) reversed the effect of IBU and indomethacin (INDO) on BACE1 mRNA levels. E, Quantification of BACE1 steady-state mRNA levels in four to five experiments performed in N2A cells. F, BACE1 activity from N2A cells is affected by treatment with similar cytokines and NSAIDs. Asterisks indicate significant differences between control and treatment. Number signs represent differences between cytokines and NSAIDs. *p < 0.05. Columns represent average ± SEM; #p ≤ 0.05; ##p ≤ 0.01; ###p ≤ 0.001; ANOVA followed by a Tukey's post hoc test. C, Control.