Skip to main content
. 2003 Nov 19;23(33):10681–10690. doi: 10.1523/JNEUROSCI.23-33-10681.2003

Figure 8.

Figure 8.

Effects of 7NI on nitrotyrosine and poly(ADP-ribose) accumulation in hippocampal CA1 neurons after hypoglycemia. A, Double-label immunostaining for nitrotyrosine (red) and PAR (green) indicated that almost all CA1 neurons immunoreactive for nitrotyrosine were also immunoreactive for poly(ADP-ribose). Panels are representative of three rats in each group. Scale bar, 100 μm. B, Neurons in layers III and IV of piriform cortex (PirCtx), which are relatively resistant to hypoglycemia, show less intense nitrotyrosine staining and barely detectable PAR staining after hypoglycemia. Sections are from the same brain as shown in A. Scale bar, 100 μm. C, Hypoglycemia increased both nitrotyrosine and PAR immunoreactivity in CA1 neurons 2 hr after glucose correction. This was prevented in rats treated with the nNOS inhibitor 7NI (50 mg/kg) administered during the EEG isoelectric period. Scale bar, 200 μm. D, Histological assessment 7 d after hypoglycemia showed substantially less neuronal death in vulnerable brain regions of the 7NI-treated rat brains. Cortex, Layer II of perirhinal cortex; DG, hippocampal dentate granule cells. n = 6-7; *p < 0.05.