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. 2003 Nov 19;23(33):10633–10644. doi: 10.1523/JNEUROSCI.23-33-10633.2003

Figure 5.

Figure 5.

Distribution of MAP2 in p35+/+ and p35-/- brains. Images of 10 μm sagittal sections of p35+/+ (A, C, E, G, I, K) and p35-/- (B, D, F, H, J, L) brains incubated with the AP18 antibody to phospho-Ser136 in MAP2B (A, B, E, F, I, J) or the AP20 nonphospho-MAP2 antibody (C, D, G, H, K, L). A, E, I, Phospho-MAP2B (AP18) stains neuronal somata (arrows) and apical dendrites (arrowheads) in p35+/+ cortex (A), hippocampus (E), and pons (I). B, F, J, In p35-/- brains, AP18 staining is diminished greatly or not detected in neuronal somata (arrows) and dendrites (arrowheads) in the cortex (B), hippocampus (F), and pons (J). Dendritic organization is disrupted (B, F), and staining is sometimes shifted to the neuropil (F, asterisk). The granule cell layer of the CA2/CA3 region of the hippocampus is disorganized in p35-/- brains (F). C, G, K, The AP20 nonphospho-MAP2 antibody stains neuronal somata (arrows) and apical dendrites (arrowheads) in p35+/+ cortex (C), hippocampus (G), and cerebellum (K). D, H, L, In p35-/- brains, AP20 staining is shifted from neuronal somata and apical dendrites to more distal processes (asterisks), and dendritic organization is disrupted in cortex (D) and hippocampus (H). Disorganization of the granule cell layer is apparent (H). In the cerebellum (L), AP20 staining in Purkinje cell somata is decreased (arrow), whereas staining in more distal dendritic arborizations is robust.