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FM1-43 label colocalizes with AChR clusters in wild-type fish. A, A 5-d-old wild-type fish colabeled with 0.1 μm Alexa 594-conjugated α-Btx and 10 μm FM1-43. The green FM1-43 labeling of synaptic varicosities (left) and the red labeling of AChR clusters by Alexa 594 α-Btx (middle) colocalize in the merged image (right). B, The same field is shown after 3 min of high-potassium treatment. The area shown corresponds to approximately one tail segment. Scale bar, 20 μm.
