Figure 3.

FM1-43 labels nerve in both wild-type and mutant synapses. Secondary motor neurons were visualized in transgenic wild-type and mutant lines of fish that were expressing cytoplasmic GFP. The GFP was constitutively expressed in the motor neurons of all three lines using a neuron-specific Islet1 promoter (top). Three Islet1 fish, corresponding to wild-type, nic-1, and sofa potato, were labeled with 10 μm FM1-43 (middle). Merged images indicate that the motor neuron GFP label colocalizes with the FM1-43 label (bottom). Scale bar, 50 μm.