Figure 6.

Destaining kinetics of FM1-43-loaded varicosities induced by electrical field stimulation. Representative time-lapse images of labeled varicosities (indicated by arrows) at 24 sec intervals after the onset of electrical stimulation are shown for wild-type (A), nic-1 (B), and sofa potato (C) fish. Scale bars, 10 μm. The time course of fluorescence loss associated with the corresponding varicosities is shown in A-C. Time 0 corresponds to the onset of electrical stimulation. Fluorescence intensity was measured every 3 sec. The time required for decay from 90 to 10% of peak fluorescence determined for each trace is indicated alongside the corresponding legend bar. In several traces, the final plateau for destaining occurred beyond the time shown in the traces.