Figure 12.

Modulatory effects of the benzodiazepine receptor inverse agonists Ro 15-4513 and FG 7142 on GABA_A receptor function in hippocampal neurons subjected to chronic exposure to or withdrawal of ethanol. A, B, Representative tracings of Cl^- currents induced by 1 μm GABA in control cells, cells exposed to 100 mm ethanol for 5 d, and cells subjected to ethanol withdrawal for 6 hr. GABA was administered in the absence or presence of Ro 15-4513 (A) or FG 7142 (B), each at a concentration of 3 μm. C, Quantitation of the modulatory effects of Ro 15-4513 and FG 7142 on GABA-evoked Cl^- currents in the three experimental groups. Data are expressed as percentage change in the GABA response and are means ± SE of values from 5-12 neurons. ^*p < 0.05, ^**p < 0.01 versus value for control cells.