Figure 13.

Effects of diazepam on the changes in GABA_A receptor sensitivity to lorazepam, flumazenil, or Ro 15-4513 induced by ethanol withdrawal. A-C, Representative traces of Cl^- currents induced by 1 μm GABA in hippocampal neurons that had been incubated first for 5 d with 100 mm ethanol and then for 6 hr in ethanol-free medium in the absence or presence of 10 μm diazepam. GABA was applied in the absence or presence of 1 μm lorazepam (A), 3 μm flumazenil (B), or 3 μm Ro 15-4513 (C). D, Quantitation of the modulatory effects of lorazepam, flumazenil, and Ro 15-4513 on GABA-evoked Cl^- currents in the two experimental groups. Data are expressed as percentage change in the GABA response and are means ± SE of values from 9-16 neurons. ^*p < 0.01 versus the corresponding value for cells subjected to ethanol withdrawal in the absence of diazepam.