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. 2003 Dec 17;23(37):11711–11724. doi: 10.1523/JNEUROSCI.23-37-11711.2003

Figure 5.

Figure 5.

Lack of effect of baclofen on changes in the abundance of GABAA receptor subunit mRNAs induced by ethanol withdrawal. Hippocampal neurons were incubated first for 5 d with 100 mm ethanol and then for 3 hr in ethanol-free medium in the absence or presence of 100 μm baclofen. Cells incubated for 5 d in the absence of ethanol were also exposed to baclofen for 3 hr. The abundance of GABAA receptor α1 (A), α2 (B), α3 (C), α4 (D), γ2L (E), and γ2S (F) subunit mRNAs was then determined by RNase protection assay. Data are means ± SE of 9-12 values from three independent experiments, each performed in triplicate or quadruplicate, and are expressed as a percentage of the corresponding value for control cultures incubated for 5 d in the absence of ethanol. *p < 0.05, **p < 0.001 versus control.