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Immunoblot analysis of the GABAA receptor α4 subunit in hippocampal neurons. A crude membrane fraction (40 μg of protein) of cultured hippocampal neurons was subjected to immunoblot analysis with antibodies specific for the GABAA receptor α4 subunit in either the absence (lane 1) or presence (lane 2) of the α4 peptide N1-19. The positions of molecular size standards and of the α4 protein are indicated.
