Table 1.
Effects of chronic ethanol treatment and ethanol withdrawal on the total RNA content of hippocampal neurons
|
|
Total RNA (micrograms per dish) |
||||
|---|---|---|---|---|---|
| Experiment
|
Control
|
Chronic EtOH
|
EtOH withdrawal
|
||
| 1 | 14.41 ± 2.03 | 12.88 ± 2.32 | 15.40 ± 3.51 | ||
| 2 | 13.49 ± 3.02 | 15.93 ± 5.95 | 12.97 ± 5.65 | ||
| 3 | 16.85 ± 1.41 | 15.05 ± 2.66 | 16.25 ± 3.53 | ||
| 4 | 14.37 ± 2.15 | 16.25 ± 2.12 | 17.23 ± 2.79 | ||
| 5
|
18.69 ± 4.28
|
16.56 ± 1.15
|
15.36 ± 3.57
|
||
Cells were cultured in 35 mm dishes for 5 d in the absence (control) or presence (chronic EtOH) of 100 mm ethanol; some ethanol-treated cells were also incubated for an additional 6 hr in the absence of ethanol (EtOH withdrawal). Total RNA was isolated from the cells and quantitated. Data are expressed as micrograms of RNA per culture dish and are means ± SE of three dishes from five randomly selected experiments.