Table 2.
Morphometric parameters of rat hippocampal neurons subjected to ethanol withdrawal in the presence of diazepam, GHB, or baclofen
|
Experimental group |
Area (μm2) |
Perimeter (μm) |
Circularity |
Maximum axis length (μm) |
Breadth (μm) |
|---|---|---|---|---|---|
| Control (n = 33) | 173 ± 9 | 51.2 ± 1.3 | 15.6 ± 0.3 | 19.0 ± 0.5 | 13.7 ± 0.5 |
| Withdrawal (n = 41) | 171 ± 12 | 51.8 ± 2.0 | 16.3 ± 0.3 | 19.0 ± 0.8 | 13.6 ± 0.5 |
| Withdrawal + diazepam (n = 23) | 158 ± 9 | 47.7 ± 1.3 | 14.6 ± 0.2 | 17.4 ± 0.5 | 13.1 ± 0.4 |
| Withdrawal + GHB (n = 25) | 169 ± 10 | 51.1 ± 1.5 | 16.4 ± 0.3 | 18.5 ± 0.7 | 13.2 ± 0.4 |
| Withdrawal + baclofen (n = 22)
|
171 ± 11
|
50.5 ± 1.7
|
15.2 ± 0.2
|
18.0 ± 0.8
|
13.5 ± 0.5
|
Cells were cultured for 5 d in the absence (control) or presence of 100 mm ethanol, after which the ethanol-treated cells were incubated for an additional 6 hr in ethanol-free medium in the absence or presence of diazepam (10 μm), GHB (100 mm), or baclofen (100 μm). The cells were then examined by confocal laser-scanning microscopy for determination of the indicated morphometric parameters. Data are means ± SE of values determined from the indicated number (n) of cells.