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. 2003 Nov 12;23(32):10433–10444. doi: 10.1523/JNEUROSCI.23-32-10433.2003

Figure 8.

Figure 8.

Overexpression of EGFP constructs with the β-actin zipcode resulted in recruitment of endogenous β-actin mRNA and ZBP1. Cultured rat hippocampal neurons were transfected with EGFP, EGFP-zipcode, EGFP-β-actin, and EGFP-β-actin-zipcode. All constructs were expressed for 48 hr. A, Fluorescence in situ hybridization to detect endogenous β-actin mRNA was performed using oligonucleotide probes that excluded the region containing the zipcode. Measurement of average β-actin mRNA levels in dendrites using digital imaging analysis showed an increase in neurons transfected with EGFP-zipcode compared with EGFP alone (*p < 0.05; Student's t test). B, Quantitative immunofluorescence of ZBP1 levels in dendrites showed an increase in both zipcode-containing constructs compared with constructs lacking the zipcode (*p < 0.05; Student's t test; histogram indicates mean values ± SEM).