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. 2003 Nov 12;23(32):10214–10223. doi: 10.1523/JNEUROSCI.23-32-10214.2003

Figure 2.

Figure 2.

The hprt-docking site is permissive and does not deregulate MBP targeting in myelinating glia. A, E, As predicted from pronuclear lines (Foran and Peterson, 1992; Forghani et al., 2001), a lacZ reporter construct driven by -3.1 kb of proximal promoter sequence and SCE1 is expressed in spinal cord (SpC) and brain oligodendrocytes and spinal root (SpR) Schwann cells of P18 mice (arrows). B, F, As observed with randomly integrated constructs, -3.1 kb-mediated oligodendrocyte expression gradually shuts off in adults, whereas high-level SCE1-mediated Schwann cell expression is maintained (shown at 6 weeks). C, G, A reporter construct driven by -9.4 kb, and thus containing the full complement of recognized evolutionarily conserved sequence, is expressed at high levels in myelinating cells throughout development and maturity (shown at 12 weeks). D, H, LacZ driven by the 0.3 kb HSP68 minimal promoter alone is expressed at high levels only in CNS endothelial cells, with no detectable expression in either oligodendrocytes or Schwann cells (shown at 6 weeks).