Figure 3.

Cell proliferation occurs in the SVZ of the adult bax^-/-bak^-/- brain. After 12 daily BrdU injections, mice were killed 1 d (acute), 12 d, or 4 weeks later. Histological sections were stained with anti-BrdU (green) and with an anti-histone-3 antibody (red). BrdU-incorporating cells were observed in the bax^-/-bak^-/- mice at increased numbers when compared with the wild-type mice (for quantitation, see Results). In addition, the bax^-/-bak^-/- mice show a relatively constant (over time) number of cells in the cell cycle as visualized by antibody to acetylated histone-3. Scale bar, 150 μm.