Figure 7.

Peripheral mechanisms are not regulating anti-nociception induced by IFD. The effects of naloxone methiodide (2 mg/kg, s.c.) on chemical visceral pain test were checked in AL and IFD mice. A total of eight animals per group was used. The statistical analysis was performed by two-tailed Student's t test. ⁺ indicates statistical significance of the same treatments between groups AL and IFD; ^* indicates statistical significance of the different treatments with respect to vehicle in the same group; ⁺⁺,^**p ≤ 0.001; ⁺⁺⁺,^***p ≤ 0.001.