Figure 1.

Expression of Rnd1 mRNA in developing rat brain. A, Total RNA (20 μg) isolated from embryonic (E14, E16, and E20) or postnatal (P0, P4, P8, P14, P20, P28, and adult) rat brain was subjected to Northern blot analysis with a cDNA probe for Rnd1. The same membrane rehybridized with GAPDH cDNA probe is shown below as the internal control. B, Distribution of Rnd1 in the P12 rat brain. In situ hybridization was performed with antisense Rnd1 riboprobe (a, c) and the corresponding sense riboprobe (b) for coronally sectioned P12 rat cortex (a, b) and hippocampus (c). Scale bar, 100 μm.