Movie S1.
Crk knockdown often leads to wide-spread nuclear loss but embryos can largely repair the epithelium before gastrulation. Time-lapse movies showing actin dynamics in control and crkS-RNAi embryos expressing Moesin::GFP to label F-actin. The Movie begins during the last syncytial nuclear division and extends until the onset of gastrulation. In the crkS-RNAi embryo, large patches of nuclear loss are apparent following the last nuclear division. During cellularization, cells at the margins of these patches can be seen exhibiting protrusive cell behavior and migrating into areas of cell loss to enclose the blastoderm embryo prior to the onset of gastrulation. Images acquired at 10 s intervals and displayed at 10 frames per second. Time between frames varies between genotypes due to different sizes of region scanned. Scan time was 2.82 seconds for the control embryo and 2.30 seconds for the crkS-RNAi embryo.