Movie S2.
Crk knockdown leads to reduced actin cap expansion and less apparent pseudocleavage furrows during syncytial nuclear cycles. Time-lapse movies showing actin dynamics in control and crkS-RNAi embryos expressing Moesin::GFP to label F-actin during nuclear cycles 10-14. In both control and crkS-RNAi embryos, actin caps form, expand, and merge into pseudocleavage furrows. However, in crkS-RNAi conditions, actin cap expansion is reduced compared to controls, leading to impaired pseudocleavage furrow formation. Images acquired every 10 s and displayed at 10 frames per second. Time between frames varies between genotypes due to different sizes of region scanned. Scan time was 2.30 seconds for the control embryo and 2.93 seconds for crkS-RNAi embryo. Focal plane was occasionally adjusted to maintain a clear view of the furrows. This video is related to figure 6K,L.