FIGURE 4:

Rtn4a levels modulate cell adhesion. HeLa cells were transiently transfected with a plasmid expressing GFP-NLS as a control, a plasmid expressing Rtn4a-GFP, or siRNA against Rtn4. Cells were allowed to attach to collagen-coated dishes for 30 min. Unattached cells were removed, and attached cells were fixed, stained with crystal violet, and lysed. Cell lysate absorbance was measured at 540 nm (see Materials and Methods). Averages from nine independent experiments are shown. Error bars represent SD. **, p ≤ 0.01; *, p ≤ 0.05.