FIGURE 6:

ZO-2 colocalization with 14-3-3σ in the cytoplasm of monolayers cultured in LC diminishes with the CS and is accompanied by a transient colocalization at the cell borders. (A) Immunofluorescence observation of ZO-2 and 14-3-3σ in monolayers cultured in the SS condition, LC condition for 20 h, or 2, 4, and 6 h after a CS. Left panel, representative images; right panel, cytoplasmic PCC. Statistical analysis done with Kruskal-Wallis test followed by Dunn’s multiple comparison test; ****p < 0.0001; ns, nonsignificant. Bars, 15 μm. (B) Fluorescence covariance index (FCI = Log10 of PCC at cell periphery/PCC at cytoplasm) was done to compare the frequency of colocalization of 14-3-3σ with ZO-2 in the cell borders with the colocalization in the cytoplasm. SS, steady state condition. The values of FCI 2, 4, and 6 h after the CS are different from the SS condition since a small but significant proportion of cells exhibited FCI > 0 indicating that in them the colocalization of 14-3-3σ with ZO-2 was higher at the cell borders than at the cytoplasm. Statistical analysis done with one-way ANOVA followed by Dunnett’s multiple comparison test, SS ≠ CS (2 h) ****p < 0.0001; SS ≠ CS (4 h) ****p < 0.0001; SS ≠ CS (6 h).