Figure 1.

Schematic representation of time course and experimental manipulations for each set of experiments in this study. Each horizontal line represents the duration of each experiment. Numerals above the horizontal line indicate time point (in days) of experimental end-points, whereas the set of five vertical lines indicates the timing of MPTP dosing (25 mg/kg measured as free base, i.p., per day for 5 consecutive days). A, Mice used for experiments examining the time course of calpain activation after MPTP intoxication were taken either before MPTP (day 0) or 7 or 14 d after the first injection (+7, +14). B, Mice implanted with osmotic minipumps (Alza) 1 d (-1) before the initiation of the MPTP regime (0). In this set of experiments, the locomotor behavior of these mice was analyzed 1 d before (+13) the 2 week end point (+14), and additional groups were also analyzed for survival at 3 weeks (+21). C, Recombinant adenoviruses were administered 1 week (-7) before the start of MPTP dosing (0), and tissues were analyzed 2 weeks later (+14). See Materials and Methods for details.