Increased calpastatin in the SNc ipsilateral to intrastriatal adenovirus administration (a), when compared with the contralateral hemisphere (b), and colocalization within dopaminergic SNc neurons (c,d). Representative coronal midbrain section at the level of the MTN (-3.16 mm caudal to bregma) (Franklin and Paxinos, 1997) from a saline-treated mouse (f) and the ipsilateral hemisphere of MPTP-treated mice that received intrastriatal administration of recombinant adenoviruses containing either lacZ (g) or the calpain inhibitor protein calpastatin (h). Images are representative of dopamine neuron survival observed 2 weeks after treatment with MPTP (n = 6 per group). e, Quantification of dopamine neuron survival per section in the ipsilateral SNc of Ad.lacZ, Ad.CALP, and uninjected-MPTP-treated or unlesioned mice. *ANOVA, p < 0.0001; Newman-Keuls, p < 0.001; Ad.CALP versus Ad.lacZ or MPTP.