Figure 1.

Identification of the purified DdlA by SDS‐PAGE (a) and WB (b) and specificity testing of the anti‐DdlA polyclonal antibody by WB (c). M, PageRuler prestained protein ladder (Fermentas). Colorimetric visualization was performed using BCIP/NBT solution in the WB analysis (a and c) and Coomassie in the SDS‐PAGE analysis (b). The monoclonal anti‐polyhistidine antibody at a 1:5,000 dilution was used to probe the expressed DdlA (a); the produced polyclonal anti‐DdlA antibody at a 1:2,000 dilution was used to probe the purified DdlA (c). a and b, 1‐2, the fractions of the purified DdlA. c, 1, purified Mycobacterium smegmatis DdlA