Table 2.
Properties of the 20 differentially expressed proteins identified by MS
| Protein Spot No. on the 2D Gel | ddlA/pMind | Protein Name | NCBI Accession No. | No. in Mycobacterium smegmatis | No. in Mycobacterium tuberculosis | MW (Da) | PI | Matched Peptide Count | Score | Protein Score CI (%) |
|---|---|---|---|---|---|---|---|---|---|---|
| 5403 | 0.42 | SAM‐dependent methyltransferase | WP_011731145.1 | MSMEG_6235 | Rv3699 | 24485.2 | 5.01 | 7 | 278 | 100 |
| 7403 | 0.05 | Heat shock protein hspX | CKI07130.1 | MSMEG_3932 | Rv2031c | 15917.1 | 4.93 | 9 | 307 | 100 |
| 2506 | 0.26 | Acetyl‐CoA acetyltransferase Fada6 (acetoacetyl‐CoA thiolase) | CKI42884.1 | MSMEG_6008 | Rv3556c | 40639.6 | 5.01 | 16 | 157 | 100 |
| 2217 | 0.11 | Carbohydrate kinase CbhK | CKI12759.1 | MSMEG_4270 | Rv2202c | 34832.4 | 4.62 | 16 | 208 | 100 |
| 1503 | 0.28 | Aldehyde dehydrogenase (NAD) family protein | ABK71967.1 | MSMEG_1665 | Rv0768 | 51884.1 | 4.9 | 16 | 169 | 100 |
| 1509 | 0.19 | Aspartyl/glutamyl‐tRNA amidotransferase subunit A | WP_003893733.1 | MSMEG_2365 | Rv3011c | 51240.8 | 4.99 | 16 | 322 | 100 |
| 1504 | 0.28 | Cystathionine beta‐synthase | CKI28531.1 | MSMEG_5270 | Rv1077 | 48750.9 | 5.03 | 12 | 281 | 100 |
| 3805 | 0.33 | Phage shock protein PspA | CKH69159.1 | MSMEG_2695 | Rv2744c | 30273.2 | 5.55 | 16 | 231 | 100 |
| 1713 | 0.18 | NDMA‐dependent methanol dehydrogenase | WP_011731151.1 | MSMEG_6242 | Rv1530 | 46262 | 5.36 | 21 | 607 | 100 |
| 2305 | 0.35 | Phosphoglycerate kinase | YP_887401.1 | MSMEG_3085 | Rv1437 | 42068.9 | 4.67 | 21 | 896 | 100 |
| 3603 | 0.05 | NAD(P)H nitroreductase | AIU23528.1 | MSMEG_5246 | Rv2032 | 36198.5 | 5.19 | 20 | 581 | 100 |
| 1607 | 0.23 | Class II fumarate hydratase | WP_011730408.1 | MSMEG_5240 | Rv1098c | 49731.8 | 5.15 | 14 | 129 | 100 |
| 4710 | 0.46 | Enoyl‐ACP reductase | YP_887466.1 | MSMEG_3151 | Rv1484 | 28508.8 | 5.28 | 13 | 406 | 100 |
| 2504 | 0.21 | Acetyl‐CoA acetyltransferase | AIU23553.1 | MSMEG_5273 | Rv1074c | 42564.3 | 4.96 | 25 | 743 | 100 |
| 5501 | 57.69 | Mitomycin antibiotics/polyketide fumonisin biosynthesis protein | CKH22930.1 | MSMEG_1419 | NA | 25674 | 5.02 | 10 | 234 | 100 |
| 1611 | 18.82 | Elongation factor Tu | YP_885786.1 | MSMEG_1401 | Rv0685 | 43708.6 | 5.18 | 21 | 1,030 | 100 |
| 1609 | 5.61 | Bifunctional o‐acetylhomoserine | WP_003893059.1 | MSMEG_1652 | Rv3340 | 46795 | 5.12 | 13 | 648 | 100 |
| 2702 | 3.21 | M18 family aminopeptidase | WP_011730828.1 | MSMEG_5828 | Rv0800 | 44851.4 | 5.25 | 18 | 196 | 100 |
| 1350 | 3.95 | Putative acetamidase/formamidase | CKI14571.1 | MSMEG_4367 | NA | 44469.1 | 4.67 | 13 | 152 | 100 |
| 1709 | 3.16 | NAD(P)H‐quinone dehydrogenase | WP_011727859.1 | MSMEG_1735 | Rv3303c | 49036.8 | 5.37 | 18 | 86 | 99.983 |
ddlA/pMind > 1, upregulation; ddlA/pMind < 1, downregulation.