Figure 3.

Homologous overexpression of DacZ and DacZD¹⁹⁴A in Haloferax volcanii H1424. Figure represents one of three biological replicates with similar results. (a) Plate sections of H1424 transformed with empty backbone control (pTA1991), 6x histidine‐tagged DacZ overexpression construct (pSVA5417) and 6x histidine‐tagged DacZD¹⁹⁴A overexpression construct (pSVA5433). (b) SDS‐PAGE of total cell pellet of H1424 cells transformed with empty backbone control or DacZD¹⁹⁴A construct (Molecular weight 6x His‐DacZ: 29.8 kDa). (c) α‐histidine Western blot of total cell pellet of H1424 cells transformed with empty backbone control or DacZD¹⁹⁴A construct. Bands appearing in both lines are unspecific bands characteristic for H. volcanii. (M: molecular weight marker)