Figure A11.

pTA1369 and pTA1451 are pyrE2‐marked vectors used to generate tryptophan‐inducible alleles of genes at their native chromosomal locus. They feature a multiple cloning site comprising a tryptophanase promotor p.tnaA in pTA1369 or a reduced activity p.tnaM3 promoter in pTA1451, an optional 6x His tag, and an hdrB marker under a ferredoxin promotor; the multiple cloning site is flanked by BglII sites, so that it can be inserted at the compatible BamHI site between the upstream and downstream flanking sequences of a gene deletion construct