pTA131 |
pyrE2 marked integrative vector for knock‐outs in Haloferax volcanii (Ampr) |
– |
– |
Allers et al., 2004
|
pTA298 |
Vector containing trpA marker under a ferredoxin promoter between two BamHI sites (Ampr) |
– |
– |
Lestini et al., 2010
|
pTA356 |
hdrB marked shuttle vector with pHV1 replication origin (Ampr) |
– |
– |
Norais et al., 2007
|
pTA409 |
pyrE2 and hdrB marked shuttle vector with pHV1 replication origin (Ampr) |
– |
– |
Delmas et al., 2009
|
pTA1228 |
pyrE2 and hdrB marked expression vector with pHV2 replication origin and tryptophanase promoter p.tnaA, for tryptophan‐inducible gene expression of N‐terminal 6x His‐tagged protein (Ampr) |
– |
– |
Brendel et al., 2014
|
pTA1369 |
pyrE2 marked vector containing a tryptophan promotor (p.tnaA)‐multiple cloning site‐construct with a hdrB marker under a ferredoxin promotor between two BglII sites; used to generate a tryptophan‐inducible allele of gene (Ampr) |
– |
– |
This study; derived from pTA131 and pTA1228 (see Figure A11) |
pTA1392 |
pyrE2 and hdrB marked expression vector pTA1228 with insertion of Strep II tag, for tryptophan‐inducible gene expression of N‐terminal 6x His‐tagged and/or C‐terminal StrepII‐tagged protein (Ampr) |
– |
– |
Gamble‐Milner, 2016 |
pTA1451 |
pyrE2 marked vector containing a tryptophan promotor with reduced leak activity (p.tnaM3)‐multiple cloning site‐construct with a hdrB marker under a ferredoxin promotor between two BglII sites; used to generate a tryptophan‐inducible allele of gene (Ampr) |
– |
ClaI; BclI; NdeI |
This study; p.tnaM3 promoter from pNPM‐tnaM3‐HfxMCM (S. MacNeill, unpublished)(see Figure A11) |
pTA1992 |
pyrE2 and hdrB marked expression vector with pHV2 replication origin and synthetic promoter (p.syn), for constitutive gene expression of N‐terminal 6x His‐tagged and/or C‐terminal StrepII‐tagged protein (Ampr) |
p.synF; p.synR2 |
BstBI; NdeI |
This study |
p7XNH3 |
Expression vector for E. coli using T7‐promotor with N‐terminal 10x His‐Tag (FX‐Cloning system) (Kanar) |
– |
– |
Geertsma & Dutzler, 2011
|
pSVA3930 |
pTA131‐based deletion construct for dacZ containing ~500 bp upstream and downstream flanking regions ligated via BamHI site (Ampr) |
8001; 8002; 8003; 8004 |
KpnI; BamHI; XbaI |
This study |
pSVA5408 |
p7XNH3‐based expression vector for heterologous expression of dacZ with N‐terminal 10x His‐Tag in Escherichia coli (Kanar) |
7644; 7645 |
SapI |
This study |
pSVA5411 |
pTA1369‐based vector containing a tryptophan‐inducible (p.tnaA) allele of dacZ with a hdrB marker under a ferredoxin promotor between two BglII sites (Ampr) |
7654; 7662 |
NdeI; EcoRI |
This study |
pSVA5414 |
pSVA3930‐based integrative vector with tryptophan‐inducible (p.tnaA) allele of dacZ with a hdrB marker under a ferredoxin promotor inserted between upstream and downstream flanking regions at BamHI site (Ampr) |
– |
BglII; BamHI |
This study |
pSVA5417 |
pTA1992‐based expression vector for homologous expression of dacZ with N‐terminal 6x His‐Tag in H. volcanii (Ampr) |
7669; 7633 |
PciI; NcoI; EcoRI |
This study |
pSVA5418 |
pTA131‐based deletion construct for predicted Operon dacZ+mscS2 containing ~500 bp upstream and downstream flanking regions ligated via BamHI site (Ampr) |
8001; 7672 |
KpnI; BamHI; XbaI |
This study |
pSVA5419 |
pTA1451‐based vector containing a tryptophan‐inducible (p.tnaM3) allele of dacZ with a hdrB marker under a ferredoxin promotor between two BglII sites (Ampr) |
– |
NdeI; XhoI |
This study |
pSVA5420 |
pSVA3930‐based integrative vector marked with trpA under a ferredoxin promoter ligated at BamHI site (Ampr) |
– |
BamHI |
This study |
pSVA5421 |
pTA356‐based episomal vector containing dacZ with ~1,000 bp upstream and downstream flanking regions (Ampr) |
7680; 7681 |
KpnI; BamHI |
This study |
pSVA5422 |
pTA409‐based episomal vector containing dacZ with ~1,000 bp upstream and downstream flanking regions (Ampr) |
7680; 7681 |
KpnI; BamHI |
This study |
pSVA5427 |
pTA131‐based deletion construct for mscS2 containing ~500 bp upstream and downstream flanking regions ligated via BamHI site (Ampr) |
7690; 7693 |
KpnI; BamHI; XbaI |
This study |
pSVA5433 |
pSVA5417‐based expression vector for homologous expression of dacZ(D194A) with N‐terminal 6x His‐Tag in H. volcanii (Ampr) |
9804; 9805 |
– |
This study |