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. 2003 Jun 15;23(12):5020–5030. doi: 10.1523/JNEUROSCI.23-12-05020.2003

Figure 5.

Figure 5.

Study of caspase-8 in rats after acute or chronic 3-NP treatment. A, Caspase-8-related IETDase proteolytic activity in striatal and cortical homogenates. B, Analysis of brain samples by SDS-PAGE followed by Western blot for the active form of caspase-8. Recombinant caspase-8 (Casp.8) was loaded in the left lane as a positive control. Note that accumulation of the p20 subunit of the active form of caspase-8 is detected only in the striatum in the acute 3-NP model at 12 hr. Ten micrograms of proteins were loaded per lane. C, Immunohistochemical analysis of caspases-8 showing low levels of immunoreactivity in the striatum in control and chronic model, whereas increased striatal labeling is observed in the acute model(arrows). Data are means ± SEM determined in 5–10 animals. N.D., Not detectable. ★p < 0.02 compared with control, ANOVA, and post hoc Scheffé F test.