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. 2003 Jun 15;23(12):5020–5030. doi: 10.1523/JNEUROSCI.23-12-05020.2003

Figure 6.

Figure 6.

Study of caspase-3 in rats after acute or chronic 3-NP treatment. A, Caspase-3-related DEVDase proteolytic activity in striatal and cortical homogenates. B, Analysis by SDS-PAGE followed by Western blot for the active form of caspase-3. Recombinant caspase-3 (Casp.3) was loaded in the left lane as a positive control. Note that accumulation of the p20 subunit of the active form of caspase-3 (seen as a 17/19 kDa doublet) is detected only in the acute 3-NP model at 48 hr. Ten micrograms of protein were loaded per lane. C, Immunohistochemical analysis of activated caspases-3 showing low levels of immunoreactivity in the striatum in control and chronic model, whereas marked increased striatal labeling in detected in the acute model (arrows). N.D., Not detectable. Data are means ± SEM determined in 5–10 animals. ★p < 0.0001 compared with control, ANOVA, and post hoc Scheffé F test.