Table 1.
Primary antisera
|
Antigen |
Source |
Species |
Designation |
Dilution |
|---|---|---|---|---|
| Phospho-ERK1/2 (Thr202/Tyr204) | Cell Signaling | Rabbit | 9101 | 1:1000; 1:400a |
| ERK1 (K-23) | Santa Cruz | Rabbit | SC-94 | 0.2 μg/ml |
| ERK2 (C-14) | Santa Cruz | Rabbit | SC-154 | 0.2 μg/ml |
| Phospho-p38 (Thr180/Tyr182) | Cell Signaling | Rabbit | 9211 | 1:1000 |
| p38 MAP kinase | Cell Signaling | Rabbit | 9212 | 1:1000 |
| Phospho-SAPK/JNK (Thr183/Tyr185) | Cell Signaling | Rabbit | 9251 | 1:1000 |
| SAPK/JNK | Cell Signaling | Rabbit | 9252 | 1:1000 |
| GFAP
|
Sigma
|
Mouse
|
GA5
|
1:1000a
|
a
Dilution used for immunocytochemistry.