Figure 4.

Spontaneous currents, AMPA receptor-mediated currents, and AMPA-mediated synaptic current activity are unaffected in lamina II neurons of the SCDH from rAAV-GFP or rAAV-GFP-Cre mice. A–C, Whole-cell patch-clamp recordings were made in spinal cord slices from C57BL/6 (untreated controls) or floxed NR1 mice that received IPIs of rAAV-GFP or rAAV-GFP-Cre. A, The frequency and amplitude of spontaneous activity (EPSCs recorded at -70 mV) are the same in untreated C57BL/6 mice or floxed NR1 mice given either viral vector. B, AMPA (10 μm) was bath-applied to spinal cord slices from untreated, rAAV-GFP, and rAAV-GFP-Cre mice. The neurons were voltage-clamped at a holding potential of -70 mV in the presence of TTX (1 μm). Bath application of AMPA evoked exactly the same response in all three groups. C, When A fibers were fully activated by dorsal root stimulation, the monosynaptic AMPA receptor-mediated primary afferent-evoked EPSC amplitudes (-70 mV) were similar in all three groups.