Figure 7.
Confocal images showing the colocolization of synaptophysin and AT1 receptor immunoreactivities in the PVN. A,B, Synaptophysin(A, red) and AT1 receptor (B, green) immunoreactivities in the PVN viewed under a confocal microscope. C, Digitally merged images from A and B. Note that the synaptophysin and AT 1 receptor immunoreactivities are colocalized in the PVN (yellow). Magnification: A—C, 800×. D—F, Higher-magnification confocal images showing that AT1-containing varicosities (green) are in close apposition to the soma or dendrites of three biocytin-filled (red) cells. Magnification: D—F, 5040×. All images are single confocal optical sections.