Table 1.
Average burst durations and frequencies of bicuculline-provoked bursts in CA3 region of the ventral hippocampus (CA3) and layers I–III of the entorhinal cortex (EC) under different conditions
|
|
Burst duration (msec) |
Burst frequency (bursts per min) |
|---|---|---|
| During bicuculline | ||
| Control (CA3) | 331 ± 47 | 6.6 ± 0.7 (n=35) |
| Control (EC) | 403 ± 35 | 6.6 ± 0.7 (n=35) |
| After separation (CA3) | 342 ± 59 | 6.7 ± 0.6 (n=15) |
| After separation (EC) | *950 ± 42 | *4 ± 0.6 (n=15) |
| + AP-5 (CA3) | *115 ± 21 | *11.5 ± 1.7 (n=15) |
| + Cycloheximide (CA3) | *200 ± 40 | *11.3 ± 2.2 (n= 8) |
| + Actinomycin D (CA3) | 346 ± 28 | 4.6 ± 0.9 (n= 9) |
| + MPEP (CA3) | 320 ± 32 | 6.8 ± 1.2 (n=12) |
| + mGluR5 knock-out (CA3) | 298 ± 67 | 5.9 ± 0.4 (n=15) |
| After bicuculline | ||
| Control (CA3) | *195 ± 66 | *9 ± 1.2 (n=35) |
| Control (EC) | *74 ± 33 | *3.5 ± 1.1 (n=35) |
| AP-5 (CA3)
|
*120 ± 42
|
*11.9 ± 1.6 (n=17)
|
After bicuculline, Data obtained 3 hr after the beginning of bicuculline washout. After separation, The values obtained immediately after separation of CA3 from the EC (Fig. 2C). Pharmacological treatments lasted from 30 min before bicuculline application until bicuculline washout; AP-5 application after bicuculline was started concomitant with bicuculline washout. Mean values ± SEM are given. Asterisks indicate significant differences (ANOVA; p < 0.05) compared with control before bicuculline. n indicates number of slices.