Figure 3.

Dendritic growth of GAD67–GFP-expressing neurons in control medium occurred only in younger cultures. A, Confocal projection of a representative GAD67–GFP-expressing neuron first imaged at EP7 (left) and again 5 d later (right). B, Another neuron first imaged at EP22 (left) and again 10 d later (right). Scale bar: (in B) A, B, 100 μm. C, TDL growth ratio of all of the neurons in our sample during a 5 d period in normal medium, plotted against the equivalent age at first imaging. A TDL ratio of 1 (dashed line) indicates no change. Note that dendritic growth was observed in neurons first imaged at EP7–EP9 (left of the vertical dotted line), but there was no change on average in neurons first imaged at later ages (right of the vertical dotted line).