Figure 5.

BDNF and KCl enhanced dendritic growth of GAD67–GFP-expressing neurons. Left column, Morphological reconstructions of representative dendritic trees of neurons before (green) and after (red) a 5 d period in culture medium supplemented by one of the following: no supplement (A), 200 ng/ml BDNF (B), 200 ng/ml BDNF and 1 μm TTX (C), 10 mm KCl (D), or 10 mm KCl and 50 μg/ml anti-BDNF (E). Right column, Cumulative histogram of TDL in each treatment group; each data point represents the fraction of neurons in the treatment group with TDL values equal to or smaller than the corresponding x value. •, TDL at day 0 (before treatment). ▵, TDL at day 5 (after treatment). Note that the rightward shift of the curves (indicating an overall increase in total dendritic length) was considerably larger in B, C, and D, compared with control. Scale bar, 100 μm (for all images).